Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells
نویسندگان
چکیده
منابع مشابه
Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells
Background Glutamine is an essential component in culture media for most of the mammalian cell lines. It is often used as an alternative source of energy by cells, along with glucose. Glutamine metabolism induces ammonia accumulation in cell culture. Elevated ammonia concentration above 2 mM has been shown to have negative impact on both cell growth and recombinant protein productivity [1-4]. I...
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ژورنال
عنوان ژورنال: Biotechnology Letters
سال: 2011
ISSN: 0141-5492,1573-6776
DOI: 10.1007/s10529-011-0809-z